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USE OF A RAPID IMMUNOCHROMATOGRAPHIC TEST FOR EARLY DIAGNOSIS OF DENGUE HAEMORRHAGIC FEVER (DHF) Featured

Le Bich Lien, Nguyen Trong Lan * Y Hoc TP. Ho Chi Minh * Vol. 7 * Supplement of No 1: 169 - 175

A rapid immunochromatographic test for IgM and IgG antibodies to Dengue virus was evaluated by using hospital admission sera of 106 patients including 71 cases of DHF and 35 cases of other diseases. The reference laboratory diagnosis was based on the results of haemagglutination inhibition test (HI) or IgMantibody-captured-enzyme-linked-immunosorbent assay (MAC-ELISA) or virus isolation. The rapid test demonstrated sensitivity of 76% and specificity of 95%. The positive predictive value (PPV) and negative predictive value (NPV) were 96.5% and 66%, respectively. In comparison with MAC-ELISA and HI tests, rapid test showed a fair agreement in the DHF diagnosis capability with k coefficient of 0.44 and 0.57, respectively. On the other hand, the rapid test allowed to distinguish between primary and secondary infection based on appearance of unique IgM strip or IgG strip (with or without IgM strip). 100% patients with primary Dengue virus infection and 86% patients with secondary Dengue virus infection were correctly classified. Predicted values are of 50% for primary Dengue virus infection and of 100% for secondary Dengue virus infection. Furthermore, the rapid test can help physicians to earlier diagnose DHF in acute phase of the disease: more than 65% of DHF cases on day 4 and about 90% of DHF cases on day 5 may be detected by this test.

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